![]() ![]() ![]() Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. In addition to the classical restriction maps - both graphic and text-based - or site usage windows, you will be able to quickly extract a sub-sequence either in a selection or between restriction sites, to create a new PCR-based fragment or synthetic adaptors. Serial Cloner will assist you in setting-up new sub-cloning projects and in preparing the electronic versions of your constructs. Version 2.5 now allows to open protein sequences, align protein sequences and reverse translate proteins using defined codon biais tables. The Collection of Features to be scanned for can be defined and modified by the user, imported and exported. All the features are displayed on the map using user-modifiable colors the features can be manually entered, imported from GenBank or automatically found after scanning by Serial Cloner. The graphic map of Serial Cloner is really Graphic as you can easily select and extract a fragment or show single, double or multiple cutter all in the same window. Since version 2.1, Sequence Features are visible in the Sequence Map. Serial Cloner also lets you build text restriction map and quickly format it to add multi-frame translation or only show single cutters for example. Numerically select fragments, find restriction sites, ORF or any nucleotide or peptide sequence, calculate Tm of selected fragments, %GC or dynamically determine the translation your selection into peptide and calculate the MW using a compact interface. Serial Cloner 2.5, handles Annotations and Features both in the sequence and in the Graphic Map and can automatically scan for sequence Features.Īll the tools you need to analyze and manipulate your sequences are available in an all-in-one-window concept. ![]() Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Import from VectorNTI multi-file format is also supported. Serial Cloner also import files saved in the Vector NTI, MacVector, ApE, DNAstar, pDRAW32 and GenBank formats. Serial Cloner reads and write DNA Strider-compatible files and import and export files in the universal FASTA format. Serial Cloner has been developed to provide a light yet powerful molecular biology software to both Macintosh and Windows users. Serial Cloner has been developed to provide a light yet powerful molecular biology software to its users. ![]()
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![]() ![]() ![]() * Trophy Store! Earn Trophies to unlock dozens of cosmetic items that let you customize your Monkeys, Bloons, animations, music, and more. * Quests! Delve into what makes the Monkeys tick with Quests, crafted to tell tales and share knowledge. Capture tiles on a shared map and compete on the leaderboards. * Contested Territory! Join forces with other players and battle for territory against five other teams. * Odysseys! Battle through a series of maps connected by their theme, rules, and rewards. * Boss Events! Fearsome Boss Bloons will challenge even the strongest defenses. * Regular updates! We release several updates every year with new characters, features, and gameplay. Enjoy endless hours of strategy gaming with Bloons TD 6! Over a decade of tower defense pedigree and regular massive updates makes Bloons TD 6 a favorite game for millions of players. Fixed bug where the boss tower doesn't spawn sometimes.Craft your perfect defense from a combination of powerful Monkey Towers and awesome Heroes, then pop every last invading Bloon! Fixed music playing twice when you lose. Fixed regrow loading up a free health point (still happens sometimes, but much less often). Normalised movement so pressing 2 movement keys at once no longer makes you move 50% faster, as a result of this the base speed has been buffed from 6 to 8, and upgrading speed now gives you 0.5 speed instead of 0.4. ![]() You can no longer brush past projectiles and avoid damage. Flowers no longer act as obstacles to projectiles. The plasma monkey damage per shot has been increased to 2 from 1. Dart & Super Monkeys are now much more accurate. Difficulties (easy, medium, hard) each difficulty has a different boss monkey which spawns in the final round, you receive less cash the higher the difficulty, and tougher difficulties have more rounds (20 in easy, 25 in medium, 30 in hard). Moabs have larger hitboxes, move at half the speed of a normal bloon and are not affected by regrow, but they gain 25 health per upgrade! MOABS! Players can now upgrade the "MOAB Health" stat to become a moab. The sun temple no longer spawns in endless mode, but sun avatars have a random chance of spawning after round 30. Endless Mode is now out of beta, starting from round 40, tower damage will slowly increase. Slowing towers such as arctic wind now slow you down to half of your current max speed, as opposed to half of the starting out speed. The AI can place road spikes and glue on the track, these have also been added to the monkey's arsenal. Bloon Abilities! Super speed, fortification and regrow amp are new abilities that can be bought and used once per round. Multiplayer! A new game mode in which one player plays as a monkey placing down towers, and the other player plays as the bloon and tries to get to round 20! ![]() Lowered range of tack shooter to actually match btd 6 ZOMG added once the moab health reaches >250ġ2. Spike nerfed to only do half the damage and hitbox has also been made slightly smaller (still subject to change)ġ1. Moab now moves at 0.65x the bloon speed instead of 0.5x, also slightly lowered cost but decreased effectiveness of speedboost on it to encourage the tanky but slow playstyleĩ. Added time limit for bloon per round to avoid AFK players (resets if the bloon dies)Ĩ. Upped super monkey cost (still subject to change)ħ. Altered abilities to be more powerful early on but they now give less and less multiplier the more they are upgradedĦ. Added option to mute music in main menuĥ. Fixed private matches not starting if players both try to join too quicklyĤ. Corrosive glue no longer does damage after you finish the roundģ. Glue projectile no longer passes through wallsĢ. Small update this time around, some features but mostly bug fixes and balancing:ġ. MAKE SURE YOU DOWNLOAD THE LATEST VERSION OF THE GAME BELOW TO PLAY WITH PLAYERS THAT ALSO HAVE THIS UPDATE. ![]() ![]() ![]() Police officers even stepped in to alleviate the chaos, but their presence didn’t produce quick results. 4Ī Walmart in Houston was a scene like no other in 2014 when some shoppers started flinging themselves on discounted television sets in an attempt to claim them. Five moments will make you shake your head in disbelief-and maybe even discourage you from ever going shopping on Black Friday! Not only do shoppers wait for hours (sometimes even days!) to get their hands on items at those long-anticipated discounts the day after Thanksgiving, some will go to extra lengths to make sure they leave the store with what they want no matter what it takes.Īs a bonus (or maybe a source of embarrassment for years to come), these shoppers often make the news or go viral on social media, giving them their 15 minutes of fame.
![]() It is sometimes hard to forget that Shure headphones are tools first and toys second. Everything sounds good and correct but again doesn't really wow you if you are just a casual listener. The SRH-440's are certainly in keeping with this tradition. Mids are Shure’s bread and butter and are well associated with what is known as "Shures House Sound". However one should remember here that the SRH-440 is designed primarily as a monitor headphone and these are traits that are preferred in music production with no one sound spectrum dominating the other.īass does not have heaps of quantity but is rich in quality it does not seem to slow the way many of its competitors do and while, as mentioned, it doesn't slam as hard as you will find on more fashion-orientated phones it still reaches satisfying levels for audio purists. The sound on the SRH-440 is very balanced and neutral if a little boring. Shure SRH-440 HEADPHONES - Sound Quality (7.5/10) We would easily recommend these headphones for use in commuting or office environments. Shure SRH-440 HEADPHONES - Isolation (8/10)Ĭlosed-back design and the circum-aural fit paired with the thickness of the housing all lend themselves to isolating well and blocking out most ambient noise. Since cable is usually the first part to wear out on audio gear it is very nice that Shure has included a detachable system (and a very good one at that, clicking into place and locking) so upgrading and replacing worn-down parts is possible with this headphone. The headband is also pleather and could do with a touch more padding but on a whole nothing to worry about. The earpads are pleather which does tend to get a touch hot over time but they can easily be replaced by the Shure SRH-840 pads which are of thicker padding and nicer quality at a reasonable price. Overall the headphones have a very sturdy feel to them and you get the impression they can take years of daily abuse just as a studio monitor should. The headphones are mainly constructed from plastic but it is of very high quality. There is nothing too flashy about them, more so they are quite utilitarian in the looks department but one thing you do notice right away is they look classy.īuild quality, as is again common with Shure, is excellent. ![]() Rather the 440s are designed for their purpose and that is as a studio or home monitor. Personally, I love the design of the Shure SRH-440's they are not designed in any way to be fashion headphones. ![]() Shure SRH-440 HEADPHONES - Looks and Build Quality - (8/10) Not too much here which is no real surprise it comes with a stereo jack adapter and nice coiled cable which is of great quality as is the twist in the connector that is found at the side of the left phone.Īlso included is a Shure branded leather style carrying pouch which is a nice addition although it doesn't really provide much in the way of protection to your headphones on the go. Shure SRH-440 HEADPHONES - Accessories - (6.5/10) The box is big and provides good protection during transit. Shure products are generally well packaged and the SRH-440 monitor headphones are no exception. Sitting at the lower end of the Shure's over-ear studio monitor series the SRH-440 features Shure’s 40mm proprietary drivers in a closed-back design shell and claims to deliver great build quality and performance from a company that is known to be one of the largest names in the audio industry Shure SRH-440 HEADPHONES - Packaging - (8/10) ![]() ![]() ![]() With logs for income and expenses, and well as pockets for receipts and other important documents, this style can help you track your spending easier than ever before. If you prefer a more hands on approach when managing your money, a journal type budget planner may be more to your liking. It also has reminders for when your bills are due. BudgetTracker – Know where your money is going with this online money management tool that can help you keep track of your transactions and bank accounts from your phone, computer or laptop.Handy charts and visuals can help you keep track of progress. BudgetSimple – A simplified and easy to use method for getting out of debt, BudgetSimple helps you manage bills, track expenses and adjust your spending to live within your means.The basic version is free to use and there is also a paid version with additional resources available. Create an easy to use budget in a matter of minutes. EveryDollar – This free app is a great resource if you’re a fan of Dave Ramsey’s methods. ![]() It puts all your financial life all in one place to make keeping on top of your money easy. – An easy to use free money management app, helps you bring all of your finances together from your account balances to your credit cards to your investments to your credit score.While some online budget planners are free and others have a small cost, here are some great resources to log your spending online. I like things that I can access online, and it makes it easier for me when I’m already on my laptop taking care of other business. When it comes to logging and keeping on top of things, I’m personally a digital girl. Here are some of the best budget planners for beginners no matter your experience or income. Easy Ways to Cut Expenses from Your Budget. ![]() ![]() ![]() When you fill out that form, there are few things you can do to improve your chances of recovery of that account. If that happens you have to verify that this account belongs to you before you can change password, and to do that you need to fill out the Microsoft account recovery form. If you try to enter the wrong password too many times, and you didn’t set up a recovery email you can lock access to your account. Recovery email is set during configuration of Office 365. Good practice to avoid having Office 365 issues with signing in, is to have a recovery email. If not, the password reset page provides you with a possibility to contact your administrator. Important thing to remember is that you will be able to change your password by yourself if the administrator in your organization allows users to change their passwords. Or if your organization have multiple Office 365 admins, you can ask them to reset your password.If you are an Office 365 administrator, you can change your password by yourself, if you set up an alternate email account, or phone number.If you are using personal Microsoft account, go to:.If you are using a work or school account, go to.Don’t lose hope, you can still regain access to your account. If you tried to enter your credentials, and it failed, even when you checked if every letter of it is correct. You should ask him to provide you with credentials that will enable you to sign in. As passwords are sensitive to small changes, you shouldn’t copy your passwords from anywhere (like textfiles, skype’s conversations), instead try to enter it manually.Īnother possible cause is that your administrator has changed your password, without informing you about that. Check if CAPSLOCK is turned on, and check the spelling of the password you try to enter. There is a chance you entered the wrong username or password. “We are unable to recognize this user ID or password” It usually occurs when you typed in the wrong login credentials and so displays the error: When you try to log into your Office 365 account, you might encounter a sign in problem. This should fix the log in issue with Office, but if it doesn’t, try the 6th method.Common Office 365 issues Office 365 issues: # 1 – Login issues Go to the File Collaboration section and uncheck the box next to Use Office applications to sync Office files that I open. Step 2: In the dialogue box select the Office tab. ![]() Now, on the left side of the pane, locate and right-click on OneDrive – Personal. Step 1: Press the Windows + E keys together on your keyboard to open the File Explorer. If the problem still exists, try the 5th method. This should resolve the issue of sign in request by Office every few second.
![]() ![]() In other words, watch this space! How does Prime Day work?Ī Prime membership is required to take advantage of all the best Prime Day deals. Last year we saw tons more brands joining the Prime Day party throughout the course of the event–and to keep things exciting, we're seeing a new brand hop in every few hours. We’ve also got a full deep-dive into Target Circle Week for your shopping pleasure. So far Wayfair, Brooklinen, Best Buy, and Walmart have all announced responses to the Prime Day deals. It‘s like Black Friday, Cyber Monday, and Labor Day all rolled into one. Best Prime Day outdoor deals : Find fire pits, patio sets and everything you need for a backyard dream sceneĪre any other retailers offering discounts during Amazon Prime Day?Īmazon isn’t the only retailer hosting a sales event as we speak.Best Prime Day Deals under $100: Not only are these deals well priced, they're also 5-star rated.Best Prime Day competitor deals : Target, Best Buy and Dyson are all in the APD game.Best Prime Day air purifier deals : Prime Day is a perfect time to save on cleaner air.Best Prime Day vacuum deals : Robot? Cordless? Bagless? You name it, it's on sale this week.Best Prime Day mattress deals: Zinus, Casper, Nectar and more are deeply discounted right now.What are some of the best Prime Day deals for 2023? The 12-piece set comes in six cheerful shades and will make not have cabinet space a little easier to swallow. ![]() If you, or someone you know, is in need of a full cookware update, Caraway has it with style to spare. ![]() ![]() CADTutorĬADTutor offers free tutorials, tips and articles for AutoCAD, 3ds Max and associated software applications. For example, you can find videos showcasing three different ways to put a frame around your text in AutoCAD, how to scale object with a reference, and many more. They also have an extensive CAD tips video gallery with clips that teach you how to perform specific tasks in AutoCAD. CadalystĬadalyst provides a wide array of AutoCAD and other CAD software tutorials, tips and training resources. To stay current with news and tutorials as they’re produced, you can follow m圜ADsite on Facebook or Twitter. You’ll also find sample CAD files and videos to accompany the HTML AutoCAD tutorials. Each set of lessons is followed by a quiz to test your knowledge. Tutorials are organized into 4 levels: beginner AutoCAD, intermediate AutoCAD, introduction to 3D and advanced topics. M圜ADsite offers free online AutoCAD tutorials for both new and experienced users with no registration needed. So, without further ado here are 15 best online free resources for mastering AutoCAD: m圜ADsite We even listed the top AutoCAD blogs in the industry to follow if you want to become and stay a top AutoCAD user and professional. And from our findings, we created a list of top free AutoCAD tutorials and tips on the Web. To help cut down on the search time, we went ahead and did the research for you. The only problem lies in sifting through search results, doing your research, and picking the right resource. With free online learning tutorials, courses, videos and other totally free learning materials, it can be easy to find the help you need. Hold Middle mouse button then hold Shift and you will pan orthogonally vertical or horizontal when you move your mouse.Learning how to use AutoCAD doesn’t have to be difficult or expensive. Hold Shift then Middle mouse button (wheel) and you will start a 3D orbit around your model as you move your mouse. Shift + Middle mouse button (wheel) will do one of two things: If you hold down SHIFT while using Trim or Extend, it will perform the opposite command, so you can issue the TRIM command, trim a few lines, then hold down shift and when you pick lines, they will EXTEND to the ‘trim’ line. It will fillet with a zero radius without changing the radius you may have previously set. If you want to FILLET or CHAMFER with a 0 distance, start the FILLET or CHAMFER command, pick your first line, hold down shift and pick the second line. ![]() ![]() Shift + Left mouse button will remove objects from your selection set providing you are selecting the objects before issuing a command. Shift + Right mouse button will bring up your OSNAPs during editing commands providing your Right Click options are set accordingly in Options > User Preferences. It provides other options as well as changing some commands. Tips & Tricks: Using the Shift Key in AutoCAD Using the Shift key in conjunction with the mouse and right click menus will greatly improve your efficiency and productivity in AutoCAD. Several delegates have commented “I didn’t know it could do that”. On AutoCAD training courses, delegates are often surprised by the versatility of the Shift key. AutoCAD – Shift for a Change (I didn’t know it could do that). ![]() ![]() ![]() So if you combine the laptop and it’s power brick, it loses a bit of the portability aspect. It is good for the kind of laptop it is, but this does mean that you will need to carry the charger around for any work lasting more than 2 hours, and that charger is huge. The Helios 300 lasted me about 3 hours on regular usage, with the backlights turned off and brightness at around 80%, while watching a live stream. Sure it’s not the best for media consumption but you wouldn’t expect that. With good colours and games looking crisp, this monitor is perfect for gaming and all daily tasks. In the Acer Predator Helios 300, you get a brilliant 1080p 144Hz monitor. You can also see the benchmark results here: The laptop handled everything we threw at it like a champ, shure eventually it hits thermal throttling, but it is genuinely a great gaming laptop. It gets pretty loud and you would be better off getting a pair of noise cancelling headphones to drown it out. But when you plug-in this puppy in and hit that turbo button, you awaken the wrath of Poseidon hitting you with a consistent barrage of monosyllabic waves. The fans on regular, battery optimised, office use are extremely quiet, making me feel like they weren’t running at all. Personally this is great and adds an extra level of upgradability. The same goes here, while the review unit didn’t come with an extra SSD pre-installed, Acer provides you with the cables and screws needed to add your own SSD. If you have read our previous review you would also notice that the storage was 256 NVMe + 1TB SSD, what we didn’t realise was that the 1TB was a SSD that can be added later. Other than the RAM being a strange 20GB, the rest of it makes sense. These specs are what you would expect as a natural upgrade, we see it as the most logical generational step from the previous Helios 300 we tested. Nvidia GeForce RTX 2060 (with 6GB GDDR6 RAM).Intel Core i7-10750H – 2.6 Ghz Hexa-Core.Here are the specific specs of the unit we got: Still keeping the same design while upgrading internals can ensure that performance is the main focus. Not that it’s become fatter, but more that thin and lights have become thinner and lighter. The Helios 300 is not as thin and light as it was when it first came out. ![]() Minimal bezels on the sides of the monitor but still quite sizable on the top and bottom, here is probably where Acer could do a bit of tweaking.Īs before you also get the pads at the bottom to lift the laptop up, along with the caution signs everywhere warning you that this system is muy caliente (a.k.a hot). The blue design elements still remain, except for the trackpad, and gives you the same gamer-esque feel here. It’s light enough and solid enough to give you all the confidence that this laptop will last you a while. The design and dimensions might be a bit dated at this point, but it’s a tried and tested formula and it works. The laptop feels just as sturdy as before. ![]() The chamfered edges around pretty much everything, even the trackpad gives a very nice premium feel. ![]() ![]() ![]() Output ( as we already added the records with the above IDs )Įrror: UNIQUE constraint failed: student. Retrieve Update Delete The most in-use type of DB’s today in technological fields is Relational Databases. Number of records added by using rowcount my_data=[(18, 'Big John', 'Four', 55, 'female'),Ĭurs=my_conn.executemany(my_query,my_data)īy using try except we can print the error message if any and that will help in debugging the script. which may not reflect the exact number of written rows as stipulated in the sqlite3 or SQLAlchemy. We can check the row id of the last inserted records by using last_insert_rowid(). None : Uses standard SQL INSERT clause (one per row). In this tutorial, we’ll go through the sqlite3 module in Python 3. My_data=[(9, 'Tes Qry', 'Six', 78, 'male'), SQLite comes bundled with Python and can be used in any of your Python applications without having to install any additional software. ![]() Recall that a SQL INSERT statement is used to insert rows into a table and a SQL SELECT query is used to return a set of rows from a table. More records can be added by using executemany() function. Augin Python Reading Time: 3 mins read A A Python sqlite3 Select Now let’s look at a Python sqlite3 Select and Insert example. Print(id) # 3 Adding multiple records / rows X=my_conn.execute('''select last_insert_rowid()''') adddata.py import sqlite3 conn nnect('library.db') cursor conn. We can check the ROWID of this added record. My_query="INSERT INTO student values(?,?,?,?,?)" Here the data will be stored in the example. We have used placeholders ( ? ) in our query and note that my_data is a tuple used for passing value to execute() method for our query. To use the module, you must first create a Connection object that represents the database. This is required to prevent injection attack. Use ? as placeholder and a provide a tuple to pass the value to query or execute() method. Always use parameterized query when the data is coming from unknown sources. ![]() |
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